FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181545
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAG10350.08.05878960-64
CTAGGCC5901.5097612E-106.58103160-64
TACTAGT12050.0035999126.56383237
GGCCTAG6807.166818E-105.92153395-99
AGCTAGG9051.8189894E-125.720527660-64
GCTAGTC9401.8189894E-115.354472115-119
GCTAGGC10051.2551027E-105.00822960-64
Produced by FastQC (version 0.11.5)