FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15046731
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC368340.24479735830992125No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG219980.14619786849382768No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA158880.10559104166878507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA148750.054.739165
CAGTGGT155500.052.2239574
AGCAGTG172650.047.2054982
GTGGTAT174700.046.278366
TGGTATC190500.042.8935477
GGTATCA190500.042.780178
GTATCAA206100.039.542089
AAGCAGT213150.037.594351
GCAGTGG258100.031.5491263
GTGAGTA50000.020.5910632
TAACCCC51150.020.267869
AGTGAGT53750.018.6186871
GGTCCGT59150.016.1878056
ATAACCC67050.015.4616138
GAGCACA180850.013.2163239
GATCGGA184000.013.10811
GTATAAC87250.012.6245846
AGAGCAC186850.012.522228
TCTTGGG136850.011.40628635-39
GTCCGTA82000.011.3257497
Produced by FastQC (version 0.11.5)