FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16077120
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC120050.025.9218315
CTCAAAC130300.024.7542279
GGCTCAA130750.024.2412537
TCTGATG130600.024.166751
TGGCTCA133050.023.9846256
GATGGCT160150.019.9259724
GCTCAAA180800.017.840028
CCTCTAG14750.015.61985450-54
CTCTAGG12700.014.2861750-54
CTAGGAT13250.013.25845650-54
TCTAGGA14850.012.41173650-54
CTGATGG291650.011.2150222
TGATGGC346350.09.9624723
CTCGGGT70650.09.51832420-24
AACACAG89650.07.934625610-14
TCGGGTG91650.07.462933525-29
TGGGAAC100450.06.981045730-34
AAACACA112350.06.741592410-14
CTAGCAC10800.06.533130650-54
CAAACAC123700.06.099741510-14
Produced by FastQC (version 0.11.5)