FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16870849
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1383570.820095064569661No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC908040.5382301744269065No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG260990.15469879435231743Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA376550.0102.087497
CTCAAAC412050.093.204269
ATGGCTC416250.092.540585
TGGCTCA430450.089.571416
GCTCAAA439650.087.3367848
GATGGCT471650.081.63834
TCTGATG488650.079.220131
CTGATGG526050.073.433172
TGATGGC548400.070.514983
CTCGGGT295850.025.20711120-24
GGTCCTC320900.023.21309945-49
GGGTGGG325250.023.07578525-29
CCTCGGG328050.023.07526620-24
AGGTCCT324550.022.88992145-49
GGTGGGA334750.022.44241725-29
AGCGACC340900.022.35062615-19
TCGGGTG336400.022.18679425-29
CAGGTCC334050.022.04928645-49
CGGGTGG353700.021.25631725-29
CCTCTAG93250.021.23266250-54
Produced by FastQC (version 0.11.5)