FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200452
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC175200.13272272797931464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT146950.070.157044
AGCAGTG159450.064.6612852
GCAGTGG172350.059.821543
AGTGGTA177200.058.546345
GTGGTAT203900.050.985256
TGGTATC206400.050.4723367
AAGCAGT213500.048.0891881
GGTATCA234900.044.3792658
GTATCAA258200.040.290989
CGCAGAG149850.014.00956615-19
GCAGAGT150600.013.82506615-19
AACGCAG156050.013.65598910-14
TCTTGGG154600.013.26368635-39
CAACGCA165900.013.15764110-14
ACGCAGA167500.012.50754515-19
TCAACGC175200.012.38523910-14
GAGCACA140950.011.3903229
AGAGCAC140600.011.1626098
CTTGGGG97950.010.71733635-39
ATCAACG216350.010.25583410-14
Produced by FastQC (version 0.11.5)