FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13855989
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC352110.25412116017124436No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC228560.1649539415771765No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG205350.1482030622281816No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA163770.11819437789680692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA152600.063.1772467
ATGGCTC158300.060.8109865
TGGCTCA159650.060.2516676
CTCAAAC167400.058.7529839
TCTGATG178750.053.8658831
GCTCAAA197150.049.7410558
GATGGCT195950.049.2731554
CTGATGG257850.037.480172
TGATGGC286800.033.8907743
CCTCTAG26650.020.20794350-54
CTCGGGT106600.017.96603220-24
CTCTAGG20700.017.46027650-54
AGGTCCT114000.016.7367145-49
GGTCCTC114450.016.73381245-49
TCTAGGA24200.014.99453150-54
AACACAG138050.014.75978410-14
CTAGGAT25000.014.45710950-54
TCGGGTG137800.014.01381825-29
ACACAGC146500.013.73152410-14
GGTGGGA142050.013.58439925-29
Produced by FastQC (version 0.11.5)