FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15210489
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT136150.049.2324374
AGCAGTG144650.047.0871052
GCAGTGG152600.044.2556843
AGTGGTA157200.042.960535
GTGGTAT206250.032.9879846
AAGCAGT218750.031.0933461
GTATCAA244000.028.6233049
GGTATCA277300.025.1081398
TGGTATC286900.023.8402587
CAACGCA137750.010.45383710-14
TCTTGGG135100.010.13624535-39
AACGCAG148450.09.66154210-14
TCAACGC169800.08.81140510-14
GCAGAGT163000.08.6573515-19
CGCAGAG174750.08.3306815-19
ACGCAGA184550.08.01314215-19
CTTGGGG86650.07.91025135-39
AACACTA57050.07.8244526
ACACTAT62150.07.76160577
CACTATA62100.07.18849478
Produced by FastQC (version 0.11.5)