FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187481
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA64800.027.3295295
CAGTGGT64450.026.9197544
GTATCAA81050.022.2059
GTGGTAT82700.021.7619746
AGCAGTG87200.020.72552
TGGTATC88400.020.521647
GGTATCA89500.020.3498528
AAGCAGT112650.016.5531791
GCAGTGG118050.014.7591343
CCCTAGT3502.8693648E-77.4059385-89
GCAGAGT68750.05.738142515-19
TCTTGGG70500.05.432706435-39
ATCCTAG7902.5731424E-85.1040196105-109
Produced by FastQC (version 0.11.5)