FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187481
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC80400.018.177665
CTCAAAC89150.016.9587049
TCTGATG87900.016.4267351
GATGGCT91700.015.5453474
TGGCTCA96000.015.1489116
CTCTAGG7450.013.528192550-54
GGCTCAA110600.013.214247
CCTCTAG9800.013.07560950-54
GCTCAAA128350.011.8914938
CTAGGAT8900.011.16238950-54
TCTAGGA9400.010.72181250-54
CTGATGG171550.08.6031892
TGATGGC214650.07.54654173
AACACAG54400.06.03481510-14
TAGGATG19350.05.506159350-54
ACACAGC66300.05.14710310-14
CTAGCAC8201.17543095E-85.09192750-54
Produced by FastQC (version 0.11.5)