FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353482
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA59850.017.6823569
CAGTGGT82800.016.0938954
CCCTTAA65600.013.5058234
AGTGGTA102550.013.3392435
GCCACTC81400.012.912658
AGCAGTG128900.010.8417932
AGCCACT105450.010.24074657
GTGGTAT141850.09.9474946
GCAGTGG144600.09.5151893
AAGCCAC120100.09.1114516
CTTAAAT111000.08.5612656
CCTTAAA111600.08.3867245
TCCCTTA116150.07.93858433
TACAAAG151950.07.86972762
AAGCAGT179300.07.66107651
GTTCCCT124650.07.5798281
TTAAATT151350.07.08746347
ACAAAGC168850.07.08205563
TGGTATC191850.07.0547727
GTATCAA198800.06.9892079
Produced by FastQC (version 0.11.5)