FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353482
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA57800.019.181199
GCCACTC75000.015.1666968
CCCTTAA64900.012.5394324
AGCCACT100050.011.6574717
AAGCCAC115650.09.9604916
GTTCCCT123000.08.1218451
TACAAAG158450.08.09087852
CTTAAAT107750.08.0848976
TCCCTTA111050.08.0421293
CCTTAAA110600.07.7463485
ATACAAA194200.06.746991
ACAAAGC174900.06.6710333
TTAAATT153400.06.6645287
CTCACTA34750.06.54631115-19
CATTAGG45650.05.7730190-94
CTACACA45000.05.759415635-39
AAAGCCA226300.05.5992945
TAAATTT170450.05.5331128
AAATTTA167900.05.18820769
GGGGCCC49550.05.17270395-99
Produced by FastQC (version 0.11.5)