FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523236
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA65800.033.588365
CAGTGGT75300.029.7347494
GTGGTAT98900.022.783076
AGCAGTG107250.021.284062
GTATCAA109400.020.6621159
GGTATCA121400.018.679038
TGGTATC122000.018.6462487
GCAGTGG123400.018.0880723
AAGCAGT144200.016.4898471
AGAGCAC103850.06.7933198
GAGCACA105000.06.3075549
TCTTGGG79000.06.01464735-39
GATCGGA136950.05.9635161
CAACGCA101900.05.19956210-14
TATCAAC108600.05.037869510-14
Produced by FastQC (version 0.11.5)