FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523236
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC98750.017.3512219
TGGCTCA100350.017.290216
GGCTCAA100100.016.8299337
ATGGCTC101900.016.320625
CCTCTAG9450.014.16923350-54
TCTGATG129550.013.3497341
CTCTAGG7000.013.16367550-54
GATGGCT138050.012.4124784
GCTCAAA143200.011.9151348
TCTAGGA8100.011.73151550-54
CTAGGAT8750.010.36639450-54
CTCGGGT39000.09.00824520-24
CTGATGG207700.08.5966582
TGATGGC232750.07.8879653
CTAGCAC7451.8189894E-105.797759550-54
GGGTGGG69750.05.573690425-29
AGAGCGT147250.05.52480278
GGTCCTC69900.05.43813645-49
TCTAGCA8602.401066E-105.3573150-54
TCGGGTG71450.05.320163725-29
Produced by FastQC (version 0.11.5)