FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14134017
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC356020.2518887588715933No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC233880.16547312770318587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA168850.059.48477
CTCAAAC190700.053.348679
ATGGCTC199800.050.8100475
GCTCAAA206100.049.187738
TCTGATG240250.041.8799441
TGGCTCA243200.041.653986
GATGGCT294150.034.634264
CTGATGG337400.030.22272
TGATGGC393550.026.2709563
CTCGGGT105700.018.5952220-24
CCTCTAG30550.017.34550750-54
TCGGGTG136950.014.57373925-29
GGTCCTC136350.014.4053645-49
CTCTAGG23100.014.399650-54
AGGTCCT141050.014.05807145-49
GGGTGGG147850.013.6259325-29
AACACAG156350.013.59406610-14
TGGGAAC146950.013.35706730-34
GGTGGGA151000.013.341681525-29
AACACGT92750.013.19744430-34
Produced by FastQC (version 0.11.5)