FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13458654
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC268570.1995519016983422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA118500.014.4587879
AGAGCAC123350.014.1237318
GATCGGA164150.010.8502121
TCGGAAG202000.09.38015653
AAGAGCA211550.08.4053767
ATCGGAA222350.08.3272522
GAAGAGC245250.07.33844856
GGAAGAG274350.06.6650535
ATCGGTG134850.06.5180043
GGTGGCT150400.06.3182879
CGGAAGA303650.06.16699274
TATCGGT147600.06.05258752
GTATCGG162100.05.76389741
TCGGTGG173900.05.6326494
CGGTGGT165550.05.6096455
TGGGGGG43001.6370905E-105.43213841
GGCTTAA38850.05.18849315-19
CAGTCAC73050.05.04601825-29
CCCTCTA76300.05.000825430-34
Produced by FastQC (version 0.11.5)