FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13458654
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG143770.10682346094936389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT169850.010.3417688
GATCGGA168550.010.2730081
TCGGAAG210200.08.4315433
AAGAGCG211800.08.3954347
GAGCGTC222200.08.1973699
ATCGGAA222450.07.96803052
GAAGAGC258850.07.25884776
ATCGGTG140350.07.08388233
GGAAGAG287900.06.50140245
TATCGGT149800.06.49337152
GGTGGCT146500.06.24114239
TAGATCT58250.06.229028740-44
CGGAAGA319100.05.91346364
GTATCGG172550.05.86069731
GGCTTAA38250.05.420600415-19
CGGTGGT185350.05.2434435
AGTGTAG71300.05.210504525-29
Produced by FastQC (version 0.11.5)