FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14936154
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG7203.0013325E-1015.0169233
TCTAGCC10606.730261E-1011.5456455
CTAGCCG10609.59335E-810.1870956
ATCTAGC11703.7609425E-89.8571964
CTAAGAG20406.813934E-87.0585298
CCCTAAG20451.6278334E-45.6323766
ACCCTAA25251.9194875E-55.4171065
TAGCATG25059.7411044E-55.17343768
TACCCTA23852.553631E-45.1378314
GTCCCTA24353.4758315E-45.0323653
Produced by FastQC (version 0.11.5)