FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15600597
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT107800.056.2318574
AGTGGTA109850.055.3790785
GTGGTAT138850.043.915826
GGTATCA143850.042.8397948
TGGTATC149400.040.525557
GTATCAA151900.039.8585749
AGCAGTG152900.039.7892882
GCAGTGG172950.035.384683
AAGCAGT185300.033.0263371
TTAGACC22800.023.3657847
TAGTTAG24100.021.806954
GTTAGAC23850.021.4315456
TAGACCC33100.015.4423668
AGTTAGA36450.014.8133345
CAACACT69400.012.9668378
AACACTA68700.012.8893769
TCTTGGG115950.010.28280935-39
GTAGTTA58600.09.2146863
GCAGAGT143850.09.07849215-19
CAACGCA147300.09.00265110-14
Produced by FastQC (version 0.11.5)