FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15600597
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC182930.11725833312661046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC133300.041.212815
TGGCTCA137000.040.782986
GGCTCAA150950.037.204957
CTCAAAC158850.036.0796479
GATGGCT159750.034.5686654
TCTGATG160800.034.2445031
TTAGACC23350.030.2188847
TAGTTAG26100.026.482494
GTTAGAC26850.026.2796546
GCTCAAA220650.025.8438788
CTGATGG274850.020.5169982
TGATGGC284250.020.1117343
TAGACCC35650.019.9945748
AGTTAGA39900.017.684435
CTCGGGT81900.013.4676520-24
CCTCTAG24300.012.74045750-54
AACACTA66200.012.6164299
CAACACT70200.012.2052398
GTAGTTA60100.012.099753
AACACAG101350.011.84954810-14
Produced by FastQC (version 0.11.5)