FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14493408
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT80450.028.6352654
AGTGGTA85650.026.9804365
AGCAGTG98650.023.5753232
GCAGTGG118050.019.882153
GTGGTAT123950.018.8755956
AAGCAGT134750.017.64481
GTATCAA151200.016.1403059
TGGTATC150500.015.6892137
GGTATCA155450.015.3285518
ACACTAT47700.07.54597668
AACACTA43700.07.4130157
CACTATA48400.06.8418939
GCAGAGT91750.05.853255715-19
CAACGCA102800.05.406115510-14
AAACACT72150.05.08859256
Produced by FastQC (version 0.11.5)