FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14493408
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA107800.015.7619077
CTCAAAC110850.015.5226999
ATGGCTC127050.013.770375
TGGCTCA126800.013.5136676
GCTCAAA137250.012.2748468
TCTGATG150300.011.5062261
GATGGCT172350.010.3601234
AACACTA43550.08.7619997
ACACTAT48600.08.2959158
CACTATA47400.07.89823449
CTGATGG240250.07.61166862
CTCTAGG17700.07.239585450-54
CCTCTAG21950.07.01853150-54
TGATGGC284900.06.5451083
CTCGGGT60550.06.372833720-24
TCTAGGA24000.05.75913150-54
AACACAG71400.05.545921310-14
AAACACT77200.05.4091156
CTAGGAT28450.05.2125750-54
GGGTGGG74800.05.197446325-29
Produced by FastQC (version 0.11.5)