FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14048530
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA126750.037.171566
GGCTCAA128800.036.468067
ATGGCTC129550.036.201335
CTCAAAC136450.034.2968989
TCTGATG147200.032.210541
GATGGCT175300.027.0411364
GCTCAAA188250.025.3949958
CTGATGG240700.019.8747082
CCTCTAG18350.018.44080550-54
TGATGGC272200.018.0237273
CTCTAGG14300.017.01761850-54
TCTAGGA17500.013.98810950-54
CTCGGGT71700.013.27508220-24
CTAGGAT18700.013.24448150-54
AACACAG93750.010.70554310-14
AGGTCCT96100.010.06906245-49
GGTCCTC96200.09.96878745-49
GGGTGGG102950.09.53903925-29
CTAGCAC11450.09.30624250-54
ACACAGC109400.09.147758510-14
Produced by FastQC (version 0.11.5)