FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13915167
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA99350.019.7106576
GGCTCAA101500.019.4350017
ATGGCTC103700.018.8838355
CTCAAAC112400.017.9982039
TCTGATG121850.015.8722841
GATGGCT134450.014.6190484
GCTCAAA161150.012.6429948
CCTCTAG16850.010.51010850-54
CTCTAGG14200.010.44361650-54
CTGATGG200550.010.1235852
TGATGGC229100.08.956283
TCTAGGA17150.08.64719150-54
CTAGGAT20400.07.97535950-54
CTCGGGT56150.07.15419520-24
AACACAG74450.06.150192710-14
GGTCCTC73950.05.724538345-49
GGGTGGG78900.05.675499425-29
AGGTCCT76600.05.601686545-49
ACACAGC82700.05.46701710-14
GGTGGGA87900.05.094390425-29
Produced by FastQC (version 0.11.5)