FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13475602
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTAT156540.11616549672511847TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTAAATATCTCGCAGAATTTTAAACAGGAGCAAAAATATGACAAACG152110.11287807401851137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA128000.055.1761325
CAGTGGT132700.052.896374
GTGGTAT173950.040.7248656
AGCAGTG173850.040.3376242
GCAGTGG199600.035.3141063
GGTATCA204000.035.1494188
TGGTATC209100.034.3609777
GTATCAA229150.031.2288159
AAGCAGT248400.028.4343591
TATCTCG95600.022.8177979
TGTGTAA97800.022.5272731
GTGTAAA120750.018.603452
GAGCACA177100.016.1790689
AGAGCAC177450.015.5791658
GATCGGA186950.015.1738861
ATATCTC155450.014.2179388
GTAAATA170300.013.4432624
TCGGAAG220650.013.0521523
GAAGAGC242300.011.7066286
TCTTGGG121850.011.51071635-39
Produced by FastQC (version 0.11.5)