FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13475602
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC198130.147028681909721No Hit
TGTGTAAATATCTCGCAGAATTTTAAACAGGAGCAAAAATATGACAAACG168190.12481075056980757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC140150.041.355595
GGCTCAA141750.041.29567
GATGGCT156800.037.1930964
CTCAAAC166400.035.2647329
TGGCTCA177450.033.392996
GCTCAAA197050.030.035288
TATCTCG96850.029.216579
TCTGATG195800.029.1873761
CTGATGG210400.028.2710232
TGATGGC220300.027.1588613
TGTGTAA106300.026.8470421
GTGTAAA129450.022.1405412
ATATCTC159650.018.2200168
CTCGGGT65950.017.1397620-24
GTAAATA173400.016.8577794
CCTCTAG21400.015.13967950-54
TGTAAAT210800.014.1743293
GATCGGA201950.013.9886811
GAGCGTC201450.013.79619
GGTCCTC89000.013.13814245-49
Produced by FastQC (version 0.11.5)