FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19500432
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA79650.027.6577037
CACTATA80600.027.2425339
ACACTAT82200.027.2378228
CAAACAC129550.017.226985
CCAAACA139750.016.6394924
AAACACT137350.016.3533386
ACCAAAC153700.015.2709273
GCTCTTA94800.013.2138784
GTAGCTC104750.012.1129841
TATTTAG122900.010.8954149
TAGCTCT144250.09.1386712
TACCAAA287750.08.7118772
CTCTTAT174900.07.9442415
TACACTC59800.07.8735747115-119
ATACCAA345650.07.61442571
AGCTCTT214300.06.55139973
CTATAGC76000.06.498378310-14
TTTAGGC79250.06.39526860-64
GTACACT81050.06.3420863110-114
CCCCTCA81350.06.318723785-89
Produced by FastQC (version 0.11.5)