FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336160
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC157130.1386095467953875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT70100.035.9479334
AGTGGTA81750.030.7370055
AGCAGTG93900.027.073722
GTGGTAT104000.024.4371156
GCAGTGG109400.023.0374263
TGGTATC118400.021.6474557
AAGCAGT130850.019.4820461
GGTATCA136300.019.0158278
GTATCAA139800.018.488259
GCAGAGT86050.06.492611415-19
TCTTGGG85700.06.199891635-39
CAACGCA96600.05.902779610-14
AACGCAG98000.05.80376210-14
CGCAGAG102800.05.504754515-19
ACGCAGA112250.05.20808715-19
Produced by FastQC (version 0.11.5)