FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16096151
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT243720.1514150805369557Illumina Single End PCR Primer 1 (96% over 32bp)
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC164560.1022356214227861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA155700.037.104057
ATGGCTC157600.035.605485
CTCAAAC172000.033.821949
TGGCTCA200100.029.1950596
TCTGATG185750.028.901811
GCTCAAA220850.026.8950398
GATGGCT212000.026.3330654
CTGATGG303050.018.4720542
TGATGGC347050.016.4596583
CTCTAGG24150.015.800776550-54
CCTCTAG31650.015.55973150-54
TCTAGGA27650.013.85275650-54
CTCGGGT87100.013.3086820-24
CTAGGAT30400.012.93119750-54
AACACAG120900.010.58825310-14
ACACAGC130800.09.74281410-14
TGGGAAC129250.09.43611830-34
TCGGGTG127550.09.40402925-29
GGTCCTC133750.09.37711345-49
AGGTCCT133750.09.32328445-49
Produced by FastQC (version 0.11.5)