FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13033166
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT70700.035.7390944
AGTGGTA75700.033.6635485
AGCAGTG99650.026.0602362
GTGGTAT100200.025.7916586
GTATCAA110950.023.8791039
GGTATCA113300.023.3202748
TGGTATC110950.023.1653257
GCAGTGG114300.022.4843063
AAGCAGT118700.022.3311771
GCAGAGT81350.06.937968715-19
TCTTGGG86450.06.528682735-39
CAACGCA99050.05.97457210-14
TATCAAC109900.05.54194510-14
Produced by FastQC (version 0.11.5)