FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13009454
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC229120.17611807536273238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT121750.060.018534
AGTGGTA123050.059.209385
GTGGTAT148400.049.483026
AGCAGTG155150.047.379882
TGGTATC163450.044.794637
GGTATCA170050.043.22548
GCAGTGG175450.041.8158343
GTATCAA180250.040.979069
AAGCAGT188000.039.1775931
TCTTGGG121300.011.79986135-39
GCAGAGT132350.011.64063615-19
CAACGCA141400.011.03818810-14
AACGCAG148400.010.35257610-14
TCAACGC168950.09.40868310-14
GAGCACA160550.09.2373389
TATCAAC171650.09.22713410-14
ACGCAGA173150.09.15549315-19
AGAGCAC171450.09.0279878
ATCAACG184500.08.60009310-14
CGCAGAG194650.08.23299215-19
Produced by FastQC (version 0.11.5)