FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12829926
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT72350.028.5585274
AGCAGTG99550.021.1202142
GCAGTGG101100.020.5099333
AGTGGTA105550.019.779965
GTGGTAT126900.016.6785966
AAGCAGT151650.014.1050551
AACACTA61400.013.2490247
TGGTATC165900.013.104927
ACACTAT66550.012.5481698
CACTATA73400.011.0828859
GGTATCA200150.011.0061718
GTATCAA219600.09.8346619
CAAACAC88150.09.0653865
AAACACT102500.08.5685986
CCAAACA116400.07.2982964
CCACTCA54600.07.1198619
ACCAAAC118600.07.0420033
CGCAGAG65350.06.98416415-19
AGGCCCC27150.06.470091345-49
GCCACTC65800.06.45500478
Produced by FastQC (version 0.11.5)