FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12829926
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA80050.019.6959537
CTCAAAC94350.017.4733419
TGGCTCA99800.016.014626
ATGGCTC100350.015.5680745
GCTCAAA120850.013.28451258
AACACTA58800.013.2233247
ACACTAT64900.011.9802688
TCTGATG145100.011.5147851
GATGGCT153750.010.5358624
CACTATA74800.010.49076759
CTGATGG181900.09.3407182
CTCGGGT38000.08.94215320-24
TGATGGC194000.08.7210173
AAACACT97650.08.4785166
CCTCGGG45400.07.770052420-24
CAAACAC97900.07.7214555
GGTCCTC49750.07.20653945-49
CCAAACA114450.07.1711994
GGGTGGG51350.06.729589525-29
ACCAAAC114900.06.6418163
Produced by FastQC (version 0.11.5)