FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15241633
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT96650.044.5469134
AGCAGTG115100.038.032772
AGTGGTA119200.036.3612255
GCAGTGG128800.033.6511843
GTGGTAT147800.029.227736
AAGCAGT157900.027.3191591
TGGTATC174550.024.9959497
GGTATCA175650.024.9227838
GTATCAA199950.022.1819849
CAACGCA113450.08.49165110-14
TCTTGGG112750.08.10986135-39
GCAGAGT123050.07.54785515-19
AACGCAG140000.06.942984610-14
ATCCAAC96850.06.1701853
TCAACGC165800.06.088411310-14
GTATCCA110300.05.8205331
ACGCAGA173050.05.77475115-19
CGCAGAG176850.05.732090515-19
CTTGGGG82200.05.48313335-39
CCAACAT126950.05.4444855
Produced by FastQC (version 0.11.5)