FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13745667
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA95900.014.789376
ATGGCTC97750.014.4358695
CTCAAAC115650.012.8860219
GGCTCAA113500.012.6229037
TCTGATG112650.012.4888041
CCTCTAG10000.011.37435850-54
GATGGCT124850.011.3604494
CTCTAGG8350.011.03553350-54
GCTCAAA168450.09.0179698
CTAGGAT11100.08.95005950-54
TCTAGGA11600.08.564280550-54
CTGATGG180700.08.167752
TGATGGC215950.07.13456253
AGGTCCT55000.05.57622645-49
GGTCCTC57000.05.50687445-49
CTCGGGT63000.05.30226920-24
GGGTGGG66150.05.180468625-29
Produced by FastQC (version 0.11.5)