FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14680143
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC213290.1452915002258493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCC25450.013.57714759
GATCGGA135300.012.3134381
AGAGCAC144100.011.4899718
GAGCACA148150.011.321649
ATCGGAA206050.09.0209632
TCGGAAG183950.08.9688563
TTTAGTC38350.08.8224178
ACCCACT54450.07.40962032
ACTTTAG60200.06.81601436
TTGTGCG101250.06.75430547
AAGAGCA263200.06.72823767
CGTTGTG105550.06.6155455
CACTTTA64800.06.44325165
AGTCACA53650.06.333217625-29
GGAAGAG300100.06.16480355
GAAGAGC304700.06.0008596
CTTTAGT64250.05.9382017
TGTGCGC118100.05.9125788
GTGCGCT132350.05.65671739
CGGAAGA306850.05.65439224
Produced by FastQC (version 0.11.5)