FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14678926
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA108300.017.6832266
CTCAAAC112300.017.6298839
ATGGCTC110300.017.4278015
GGCTCAA118000.016.473667
TCTGATG119400.016.325221
CCTCTAG11050.014.33274550-54
GATGGCT149700.013.1297984
CTCTAGG9650.013.12968550-54
GCTCAAA151100.012.9124098
CTAGGAT12200.011.5655250-54
TCTAGGA12050.011.47052150-54
CTGATGG229150.08.98566152
TGATGGC273550.07.8167043
CTCGGGT65500.06.74843620-24
AACACAG71800.06.437454710-14
GGGTGGG84800.05.36557525-29
ACACAGC91600.05.21886710-14
CTAGCAC6351.1407938E-65.21498550-54
GGTGGGA90400.05.12876125-29
Produced by FastQC (version 0.11.5)