FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17258493
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT83100.010.5746624
AGTGGTA93350.09.71779255
ACACTAT52000.08.4452798
AACACTA54400.08.0726937
CACTATA57200.08.0551099
AGCAGTG122100.07.78755662
GTGGTAT130350.07.0694636
AAGCAGT158250.06.52364871
GCAGTGG136750.06.4791913
GTATCAA173500.05.60172469
TGGTATC171450.05.50074247
AAACACT91800.05.4896276
GGTATCA171700.05.3669458
CAAACAC99350.05.3625985
Produced by FastQC (version 0.11.5)