FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13873563
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA55450.027.3934788
CACCACT63450.024.620367
CCACTAC70850.021.8456469
ATACAAC106850.018.3011051
ACAACAC92100.016.6640623
CCACTCA37000.015.7597589
ACACCAC97700.015.5472716
TACAACA133700.014.8204772
CCTAGAC20700.014.468225120-124
GTTACGT41300.012.5436921
CTAGACC24800.012.076301120-124
ACTCTAG26750.011.948832510-14
AACACCA133600.011.4234915
ACGTTAA42950.011.4035644
GCCACTC52400.011.4028418
GGTCCTA28600.011.07621645-49
TACGTTA47000.010.8848233
CTAGAGC30350.010.6264415-19
CAACACC150450.010.5323524
ATCCTAG29500.010.444893115-119
Produced by FastQC (version 0.11.5)