FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13873563
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA56100.030.4124418
CACCACT66250.026.2966237
CCACTAC73250.024.3752449
ATACAAC107500.021.1727181
CCACTCA39400.019.18669
ACAACAC94250.018.8042583
ACACCAC100250.017.8808026
TACAACA135100.016.7472112
GTTACGT41550.016.1011731
GCCACTC51700.015.1775528
CCTAGAC22050.014.88898120-124
ACGTTAA48150.014.0608084
TACGTTA50900.013.7295533
AACACCA138650.012.9286015
ACTCTAG28400.012.72631110-14
CTAGACC27650.012.7067175120-124
AGCCACT66850.011.8457287
CAACACC156350.011.6547074
AACACTA45950.011.59343058
ATCCTAG30750.011.425937115-119
Produced by FastQC (version 0.11.5)