FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13447507
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA130750.016.5910131
TCGGAAG155200.014.2040943
ATCGGAA171750.013.0048982
AGAGCGT171350.012.6037418
AAGAGCG202950.010.9250647
GAGCGTC223350.09.7663789
GGAAGAG235300.09.3341395
GAAGAGC259950.08.613346
CGGAAGA262100.08.4932774
AGTGTCA59950.07.588935425-29
AGAGTGT59500.07.57373925-29
TAGGGAA61650.07.35629515-19
GAGTGTC65150.07.115811325-29
TAGATCT66000.07.06788640-44
GTAGGGA67200.06.962989315-19
TCATTAA68400.06.756476465-69
AAGAGTG74150.06.329791525-29
TGTAGGG78150.05.96894715-19
TCGTGTA79350.05.897125710-14
GTGTCAG79350.05.878696425-29
Produced by FastQC (version 0.11.5)