FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534757
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA51550.019.2714827
ACACTAT55550.017.7542048
CACTATA60800.016.6947639
AAACACT83800.012.1985746
CAAACAC89800.011.54398255
ACCAAAC107550.09.9826323
CCAAACA109550.09.665194
GCCCTTA58750.08.45953754
TAGCCCT63800.07.45405242
TACCAAA179700.06.41565662
TATTTAG95400.05.6594949
ATACCAA208000.05.58146331
CCCTTAT98200.05.49818475
CTATAGC45550.05.247040310-14
CCTCTCA49100.05.219691385-89
CCTTATT125000.05.12559946
GTAGCCC90750.05.06883571
Produced by FastQC (version 0.11.5)