FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534757
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA52850.019.8873167
ACACTAT55050.019.4846428
CACTATA59850.017.5624169
AAACACT88350.012.0594276
CAAACAC96550.010.9606565
ACCAAAC108800.010.2650153
CCAAACA109400.09.6127534
GCCCTTA63500.08.7343234
TAGCCCT66400.07.2716962
TACCAAA177250.06.5865472
TATTTAG95550.06.17843879
ATACCAA208100.06.01425171
CTATAGC43750.05.496409410-14
CCCTTAT104400.05.30959945
CCTCTCA50950.05.11525285-89
AGCCCTT110100.05.03918363
GTAGCCC89750.05.0266941
Produced by FastQC (version 0.11.5)