FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13531087
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG46850.028.2842925
CCCCTTA47050.027.5656594
CCTTAGA58800.022.4075956
TTAGAGC59850.022.0122018
CTTAGAG59900.021.753467
TAGAGCC59800.021.1889239
CACACAA63350.017.2883074
GTGCACA66750.016.6836281
TGCCCCT94000.014.4891882
GCCCCTT93150.014.2328043
GCACACA77600.013.8350283
TGCACAC86300.012.6923752
CACAAAC97400.011.4576156
ACACAAA118300.09.8620535
GATCGGA115150.09.7975651
ACAAACT125600.09.1707937
AAACTCA125100.09.0927919
ACATAAG78850.09.0463791
CAAACTC136250.08.4539578
CATAAGT80350.08.4304752
Produced by FastQC (version 0.11.5)