FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16700134
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG45000.016.9583429
GTGAGTA54650.016.481042
AGTGAGT53100.016.3144781
ATAAGGG102450.08.2213498
TGAGTAT123750.08.0342963
GAGTATA125550.07.97651774
GTATAAG132750.07.10479356
TTCTAGG31000.06.78158150-54
CTAGGAG30000.06.47966150-54
AGCATGA183800.06.3062389
TATAAGG149900.06.14720347
GTTCCTT224100.05.8147611
CCTTCAG211200.05.62866354
CAGCATG227550.05.409898
CTTCAGC236750.05.2622445
TCTAGGA41600.05.19203650-54
Produced by FastQC (version 0.11.5)