FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14790205
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC168470.11390646715173997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTC135050.022.0696984
TAATTCA76700.014.8303845
TACCCTA23950.012.9256854
CCCTAAG23150.012.7503916
CTAAGAG25350.012.4958378
CATATAA110300.011.9017051
ATTCTAG12306.002665E-1111.1208797
AATTCTA73100.010.6364586
ACCCTAA30150.010.2676685
TAATTCT104850.010.2307745
CCTAAGA31050.09.9700557
TCAATTC141350.09.77903654
TGCATAA104400.09.2396871
TATAATT143550.08.9773443
TAATTCG57100.08.5736055
AATTCAT149850.08.5036856
GTCAATT88450.08.4651273
CATAATT124600.08.4359263
CAATTCA92350.08.419335
GCATAAT125350.08.3859062
Produced by FastQC (version 0.11.5)