FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20006972
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC86650.019.2759258
CCACTCA87900.019.1656179
AACACTA86100.015.7198497
ACACTAT91050.015.1025128
AGCCACT132350.013.1095197
AAGCCAC135150.012.3585266
CCGTTAA99200.011.6867474
CGTTAAA114000.010.7992765
CACTATA126050.010.7376899
AAACACT133250.010.2114946
CAAACAC139700.09.9463635
TCCGTTA136400.08.8714113
CCAAACA163150.08.6506434
GTTAAAT141550.08.5446476
ACCAAAC167450.08.5168283
ACAAAGC238850.08.1119423
TACAAAG243300.07.77031762
GGGGCCC51650.07.2758155105-109
TAGCCCT83350.07.1854192
CTCACTA51750.07.06679515-19
Produced by FastQC (version 0.11.5)