FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14248630
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT37900.034.5249751
GTGAGTA46500.028.1973482
TAACCCC47000.028.181779
GTCTCTT80900.016.8971161
ATAACCC88200.015.4255818
GAGTATA96250.014.4361254
TGAGTAT101550.013.1241893
GTATAAC108200.012.308156
CAGCATA144350.012.1181928
TATAACC115450.011.78463657
AGCATAT157750.010.9519199
CTCTTCA148400.09.5634153
CGTTTAG30350.09.4947163
TCTTCAG160350.09.2938454
CTTCAGC208750.09.1728335
TCAGCAT201050.09.0944817
TACACTA32050.08.53500658
AGTATAA177750.08.3831855
TCTCTTC176100.08.3456592
GTTTAGA38300.08.2708084
Produced by FastQC (version 0.11.5)