FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14248630
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT39550.032.218691
GTGAGTA44850.028.4373592
TAACCCC49250.025.5822169
ATAACCC87550.015.2121268
GTCTCTT85700.014.7837551
GAGTATA97250.013.3347554
GTATAAC100700.013.1547926
TGAGTAT100700.013.0924893
TATAACC114550.011.8779387
TACACTA33900.011.04276858
CAGCATA149700.010.8682838
CTACACT34950.010.7110127
AGCATAT160500.010.2274229
CGTTTAG31950.09.9216173
GTTTAGA37200.09.2960934
CTCTTCA147200.09.1523883
TCAGCAT200800.08.53272157
TCTTCAG154400.08.3523354
CTTCAGC206050.08.1429955
AGTATAA171950.07.8314155
Produced by FastQC (version 0.11.5)