FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15096539
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA76100.037.8449445
GTATCAA78450.036.61959
CAGTGGT81550.034.874314
GTGGTAT86300.033.6222466
GGTATCA89800.032.1514478
TGGTATC91400.031.5886237
AGCAGTG120300.024.179552
AAGCAGT136600.021.610541
GCAGTGG154650.019.0882643
GCAGAGT73150.08.28762815-19
TATCAAC81750.07.62715610-14
TCTTGGG80250.07.159626535-39
CAACGCA97700.06.514636510-14
AACGCAG107550.06.118828310-14
CAGAGTT44700.05.18657715-19
Produced by FastQC (version 0.11.5)