FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15096539
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC82250.029.4127679
ATGGCTC93400.025.516065
TCTGATG93450.025.1171761
GCTCAAA112300.021.6705258
GATGGCT113600.020.535214
TGGCTCA113700.020.4538256
GGCTCAA131600.018.5471137
CCTCTAG9950.015.34070450-54
CTCTAGG6900.012.93913150-54
TCTAGGA7300.012.42739750-54
CTGATGG215550.011.2567852
CTAGGAT8600.011.05116450-54
TGATGGC283800.09.1585623
AACACAG56300.09.15666110-14
CTAGCAC7350.08.03265350-54
CTCTAGC8300.07.980722450-54
TCTAGCA8500.07.623529450-54
GTGGGAA69000.07.095652625-29
TGGGAAC71650.06.93370630-34
ACACAGC78350.06.74511810-14
Produced by FastQC (version 0.11.5)